VEGFA and breast cancer: TNBC, in particular, has a significantly increased deposition of collagen as well as increased matrix stiffness compared with luminal breast cancer subtypes.45 Previous studies have identified an association between increased Col1 fibers and metastasis in breast46 and prostate cancer.47 Here, SHG microscopy together with immunohistochemistry and molecular analysis clearly demonstrated that VEGF overexpression reduced Col1 fibers, protein and mRNA.