Instead, treatment of IFNγ neutralizing antibody during the coculture reduced ICAM-1 expression on target cancer cells and suppressed the killing function of CAR-T cells (Figures 4L, 4M), indicating that CAR-T cell cytotoxicity is dependent on the production of IFNγ and subsequent ICAM-1 expression on their target cells (48). This evidence concerns the gene ICAM1 and cancer.