As for TMB, there was a significantly negative correlation between HIC1 expression and TMB in multiple cancer types, including THCA, STAD, SKCM, PRAD, PAAD, LUSC, LUAD, LIHC, KIRP, HNSC, DLBC, COAD, CHOL, CESC, BRCA, and BLCA, whereas a significantly positive connection in LGG, SARC, and THYM (Figure 9A). This evidence concerns the gene HIC1 and cancer.