MGMT and neoplasm: To obtain a quantitative and spatially resolved assessment of MGMT protein expression, DNA damage response (DDR), and TILs in pretreatment and posttreatment tumors, we studied the biopsy samples with a 5-colored multiplexed QIF panel containing the markers DAPI for all cells, cytokeratin for tumor cells, MGMT, γH2AX as a marker of DDR, and CD8 for cytotoxic T cells (representative example: Fig. 3A).