However, in isolated oligohydramnios cells, the p-GSK-3β protein expression was not affected by Tanshinone IIA, and the AQPs protein expression was not affected by LiCl, suggesting that the regulation of AQPs expression by Tanshinone IIA in the amniotic epithelial cells of isolated oligohydramnios is independent of GSK-3β. The gene discussed is GSK3B; the disease is oligohydramnios.