Therefore, Tanshinone IIA reduced AQP1 and AQP3 protein expression in hAECs from normal AFV pregnant women by inhibiting the activity of GSK-3β, but the effect of Tanshinone IIA on the expression of AQP3 and AQP1 in hAECs with isolated oligohydramnios were not associated with GSK-3β signaling pathway. This evidence concerns the gene GSK3B and oligohydramnios.