In our study, “Central carbon metabolism in cancer”, “Protein digestion and absorption”, “Alanine, aspartate and glutamate metabolism”, “ABC transporters”, and “Aminoacyl-tRNA biosynthesis” pathways in HTR-8/SVneo cells were widely affected by CAMK4-overexpressing, which indicated that CAMK4 may activate metabolism of amino acid, lipid and carbohydrates in IR trophoblast cells. The gene discussed is CAMK4; the disease is cancer.