Thus, to explore the unknown function of KIF5A in astrocytes and its potential role in ALS, we utilised perturbation experiments in combination with cytoskeletal analyses, biochemical assays and super-resolution structured illumination microscopy (SR-SIM) in human induced pluripotent stem cell (iPSC)-derived and mouse astrocyte culture platforms. The gene discussed is KIF5A; the disease is amyotrophic lateral sclerosis.