The results showed that most of the steps were highly activated in Cluster 2, especially cancer antigen presentation (Step 2), immune cell recruitment such as CD4+ T cell, CD8+ T cell, dendritic cell, macrophage, NK cell and B-cell recruiting (Step 4), infiltration of immune cells into tumours (Step 5) and killing of cancer cells (Step 7). The gene discussed is CD4; the disease is cancer.