AR and prostate adenocarcinoma: NK1R was highly expressed in NE-like cell lines PC-3 and DU145, both positive of NE markers (CgA and ENO2) and negative of AR/PSA; in contrast, in AR-positive prostate adenocarcinoma cell line LNCaP and 22Rv1, NK1R expression is much lower and NE markers are marginal (Fig. 2C).