Following exposure to siCDK1‐DPPC:EVs, the mRNA levels of CDK1 in Sk‐hep1 cells exhibited an 86.7% reduction compared to controls, which was 1.7‐fold more efficient than siCDK1‐DPPC liposomes.[172] It has been reported previously that effective suppression of the CDK1 gene in c‐Myc‐overexpressing HCC cells is lethal, leading to cancer cell growth inhibition.[219] This was corroborated by the flow cytometry analysis in which Sk‐hep1 cell exposure to siCDK1‐DPPC:EVs resulted in 80.5% apoptotic efficiency, approximately twice as high as those of siCDK1‐DPPC liposomes. This evidence concerns the gene CDK1 and hepatocellular carcinoma.