To further confirm whether circRPS5 plays an inhibitory role in OS by up-regulating NPTX1 expression, we transfected NC, pc-NPTX1, sh- NPTX1, pc-NPTX1 + sh-circRPS5, and sh-NPTX1 + lv-circRPS5 into melanoma cells. This evidence concerns the gene NPTX1 and melanoma.