In vulvar melanoma, mRNA transcripts for BUB1, NME1, RRM2, CCNE1, and KPNA2 were upregulated and identified as target genes for two or more miRs with significantly decreased mean expression, including miR-494-3p, miR-200b-3p, and miR-200a-3p (Fig 6A). This evidence concerns the gene NME1 and vulvar melanoma.