LRRK2 and Parkinson disease: To investigate the role of PD-associated LRRK2 mutations on mitochondrial structure and function, we generated primary cortical neuronal cultures from LRRK2 BAC transgenic rat pups (P1–P5) expressing the human LRRK2hWT, LRRK2G2019S or LRRK2R1441C transgene, and non-transgenic (nTG) littermates (21,31).