Hence, we determined as surrogate marker of DSB, induced by Trabectedin, the phosphorylation of H2AX (ɣ-H2AX), and investigated whether this event led to the formation of RAD51 foci, which is regarded as a functional assay for testing the activation of homologous recombination (HR) in ovarian cancer (Gerić et al., 2014; van Wijk et al., 2022; Blanc-Durand et al., 2023). The gene discussed is H2AX; the disease is ovarian cancer.