For this purpose, we utilized two CC cell lines which are the first from adenocarcinoma (HeLa cells with PIK3CA wt) and the latter from cervical squamous cell carcinoma (OV2008) with mutations in PIK3CA, which are reported to be associated with an immunosuppressive microenvironment (Walsh and Tan, 2021). This evidence concerns the gene PIK3CA and cervical squamous cell carcinoma.