The cells were further annotated as specific cell type subpopulations according to the expression of classic markers, including T cells (CD3D+), myeloid cells (CD68+), endothelial cells (CD34+), hepatocytes (ALB+), B cells (CD79A+), and cancer-associated fibroblasts (CAFs) (ACTA2+) (Figure 1B; Supplementary Figure 1E, F). The gene discussed is CD34; the disease is cancer.