To verify the predicted results of network pharmacology and to find the signaling pathway related to the EPI treatment of adriamycin‐induced nephropathy, we first measured the levels of protein and mRNA associated with the PI3K/AKT pathway, and the results showed that EPI could significantly increase the levels of PI3K and p‐AKT in adriamycin‐induced nephropathy rats (Figure 5D,E). The gene discussed is AKT1; the disease is kidney disorder.