Enzyme-Linked Immunosorbent Assay (ELISA) result showed that the level of IFI35 was significantly higher in the supernatant of IFI35-overexpressing cells, and lower in the sh-IFI35 cells, compared to control cells in both murine colon cancer cell models (Fig. 4A). Here, IFI35 is linked to malignant colon neoplasm.