On the other hand, a recent study showed that knocking out SMCHD1 in HCT116 colon carcinoma cells leads to DUX4 derepression without changes in DNA methylation or H3K9me3 levels at D4Z4 suggesting that SMCHD1 is not required for the maintenance of these epigenetic marks in this cell line41. The gene discussed is SMCHD1; the disease is colon carcinoma.