Twenty-four hours after stroke, cells from subcluster 5 upregulated pathways related to downstream Myc targets, tumor necrosis factor (Tnfα) via nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), interleukin-2 (Il2)/signal transducer and activator of transcription 5 (Stat5), mammalian target of rapamycin complex 1 (Mtorc1), hypoxia, apoptosis, unfolded protein response, glycolysis, and pathways related to inflammation. The gene discussed is TNF; the disease is stroke disorder.