In our study, intraperitoneal injection of TAA at a dose of 100 mg/kg, three times per week for six consecutive weeks, was selected as a model of HE to mimic the pathophysiological events associated with human HE, starting from increased ammonia levels, oxidative stress imbalance evidenced by elevation in MDA, and reduction in GSH and SOD levels indicating excessive production of reactive oxygen species (ROS) with an elevation lipid peroxidation in brain cells. Here, SOD1 is linked to hereditary elliptocytosis.