Furthermore, a number of phosphoproteins affected by CDK8/19 inhibition in 293 cells (such as OGFR, MED14, RREB1, TP53BP1, NELFA, AFF4, BRD9, TAF10, CHD3 and STAT1) were previously identified as likely targets of Mediator kinase in HCT116 colon carcinoma (46). This evidence concerns the gene AFF4 and colon carcinoma.