To further determine the clinical significance of HK2-mediated IκBα T291 phosphorylation, thereby promoting the expression of PD-L1 in breast cancer patients, we performed immunohistochemistry (IHC) analyses of 220 breast cancer specimens with a specificity-validated anti-IκBα T291antibody and antibodies against HK2 and PD-L1 (16). This evidence concerns the gene HK2 and breast carcinoma.