We have previously validated scFEA on glycolysis and TCA cycle pathways by applying the method on our in-house-generated scRNA-seq data of 88 patient-derived pancreatic cancer cell lines, Pa03c, and matched metabolomic profiling under two conditions: knockdown of APEX1 (APEX1-si) and scrambled control (sc) under hypoxia (14). This evidence concerns the gene APEX1 and familial pancreatic carcinoma.