IFNG and neoplasm: To quantify the number of surviving or dead tumor cells in a tumor cell killing assay, we established K9TCCnRFP cells expressing nuclear-restricted RFP (nRFP) and confirmed the expression of endogenous cPD-L1 protein and mRNA in both K9TCC parental and K9TCCnRFP cells upon IFNγ treatment (Fig. 5J–L).