For example, five proteins (CD49E, CD90, CD73, CD44, and CD13) were previously identified as candidate cancer-associated fibroblast markers in serous ovarian cancer using this screening strategy (26), but here we found all proteins were expressed by all subpopulations at greater than 80% positivity (Supplementary Table S1), supporting the need to profile distinct subpopulations within mesenchymal tumors. This evidence concerns the gene NT5E and ovarian serous adenocarcinoma.