Moreover, Liu et al. reported that CDDP-resistant osteosarcoma-derived EVs contain high levels of CCCTC-binding factor, which is transferred to osteosarcoma cells, enhances IGF2-AS transcription, and increases CDDP resistance in OS cells by regulating the IGF2-AS/miR-579-3p/MSH6 axis and activating an autophagy-dependent pathway [52]. This evidence concerns the gene CTCF and osteosarcoma.