Western blotting demonstrated the presence of S2 protein, as evidenced by Figure 3C. The findings demonstrated that the expression level of CTSB significantly increased (p < 0.001) following the expression of S2 protein and subsequent infection with rSczy3, and that CTSB was significantly raised when infected with rSczy3 alone (p < 0.05), while there was no significant change in the group that had only expressed S2 protein. This evidence concerns the gene CTSB and infection.