Next, we aimed to validate our findings regarding the antiviral activity of TRIM16 overexpression in HEK293T cells by investigating additional cell lines, including A549 (human airway epithelial), HeLa (human cervical carcinoma epithelial), Hep2 (human carcinoma epithelial), and Vero (epithelial cells from African Green monkey kidney which are deficient in the production of type I IFNs) [32,33] cells. This evidence concerns the gene TRIM16 and carcinoma.