In the current study, the major compounds obtained from H. sabdariffa L. methanolic extract exhibited good interaction with the crystal structure of E. coli MenB: OSB-NCoA (PDB ID:3T88) and structure of the cyclophilin -CeCYP16(PDB ID:2HQ6) of the colon cancer that were recovered from the protein data bank. Here, PPIB is linked to colonic neoplasm.