In this, study a digital, cell-based technique was used to analyze the immunohistochemistry hypoxia markers (hypoxia-inducible factor 1-alpha (HIF-1) and and pimonidazole (PIMO) in laryngeal squamous cell carcinoma (LSCC) and their influence on prognosis in patients with laryngeal cancer treated with accelerated radiation with or without carbogen breathing and nicotinamide (AR vs. ARCON) [37]. Here, HIF1A is linked to laryngeal carcinoma.