To explore the function of COX5A in DOX-induced cardiomyopathy, we specifically overexpressed COX5A in myocardium via a tail vein injection of recombinant adeno-associated virus serum type 9 carrying COX5A (AAV9-COX5A) and negative control (AAV9-NC) before DOX treatment. The gene discussed is COX5A; the disease is cardiomyopathy.