It seems that bupivacaine significantly inhibited CRC cell vitality, promoted the apoptosis rate and expression of apoptosis genes such as Bax and caspase-3, inhibited Bcl-2 expression, prevented cancer cell migration, increased autophagy-related protein LC3B II/LC3B I ratio and Beclin-1 expression and hindered p62 expression. Here, SQSTM1 is linked to colorectal carcinoma.