EGFR and neoplasm: The CTCs observed were: 18 CTCs (tumor astrocytes, GFAP+ and/or EGFR-Ki67+), 86 nucleated hematopoietic (nH) cells (non-tumoral cells, CD45+), 12 double-positive cells (dposCTCs) (GFAP+/CD45+ or EGFR-Ki67+/CD45+), and 158 unstained cells (only Hoechst+).