In this study, we constructed novel bifunctional affibody molecules (ZLMP110-277 and ZLMP277-110) with the potential for dual binding to the target proteins LMP1 and LMP2, antitumor effects on NPC cells, and in vivo evaluation of targeted therapy for NPC in xenograft tumor-bearing nude mice. Here, PSMB9 is linked to nasopharyngeal carcinoma.