In the present in vivo investigation, JZP327A counteracted NTG-induced trigeminal hyperalgesia and the activation of pain and inflammatory pathways (CGRP, TNF-alpha, and IL-6) in the TG and medulla-pons, without affecting NTG-related hypomotility and anxiety behaviours [15] as well as an NTG-induced increase in CGRP serum levels. Here, IL6 is linked to Anxiety.