While HFD had negligible impact on plasma Gal-4, tMCAO resulted in increased Gal-4 plasma levels, as measured 3 days after stroke (Figure 1b; ANOVA effects: diet F(1,11) = 5.00, p = 0.047, stroke F(1,11) = 15.50, p = 0.002, interaction F(1,11) = 0.081, p = 0.781). This evidence concerns the gene LGALS4 and stroke disorder.