By using a combination of flow cytometry and Western blots, we were able to confirm that the hTERT promoter was able to drive the expression of hCD40L and h4-1BBL in SKOV-3 human ovarian cancer cells and in A549 human lung cancer cells, both expressing endogenous 4-1BBL (Figure 3B,C). This evidence concerns the gene CD40LG and ovarian carcinoma.