(1)RNA of acceptable quality and quantity can be obtained from DWC specimens and used in RNA-based pre-operative diagnosis of breast carcinoma.(2)CART may serve as a biomarker of DCIS as CARTPT mRNAs were detected in all samples (as for HPRT-1 mRNA—a representative housekeeping gene. This evidence concerns the gene HPRT1 and ductal breast carcinoma in situ.