EGFR and non-small cell lung carcinoma: In particular, minor EGFR mutations with a VAF <1% were identified additionally in the Oncomine Lung cfDNA Assay involving five patients, even though multiple EGFR mutations are a rare event with a frequency of <1% (446 of 46,679 patients), which suggests that a majority of NSCLC patients carry single EGFR mutations in clinical encounters [32].