Interestingly, in three patients (D070, D453, and D076) carrying no EGFR mutations at initial diagnosis in the tumor sample, different EGFR but also PIK3CA and/or TP53 mutations were detected in the cobas® EGFR Mutation Test v2 and/or the Oncomine Lung cfDNA Assay in the follow-up plasma samples. Here, PIK3CA is linked to neoplasm.