In addition, we further studied the impact of promoter sequence methylation levels on the expression of two candidate genes (FHIT and PIAS1 genes) by using pyrosequencing, RT-qPCR, etc. (The FHIT gene as a candidate gene associated with mastitis resistance was screened in Xinjiang brown cattle based on our previous research [16]). This evidence concerns the gene PIAS1 and mastitis.