Chondrocyte cells were treated for 24 h with increasing concentrations of AMC3 (0.1–100 μM), and a slight reduction in cell viability (by about 10–15%) emerged only at the highest AMC3 concentrations (30–100 μM), as shown in Figure 2A. In order to model RA in vitro, chondrocytes were stimulated with IL-1β, and firstly, the effect of treatment with different concentrations of IL-1β (1–30 ng/mL) on cell viability was evaluated. This evidence concerns the gene IL1B and rheumatoid arthritis.