We constructed, for the first time, a PRDX6 knockout cell line in mesenchymal SNU475 hepatocarcinoma cells that, together with a previously constructed PRDX6 knockout in the more epithelioid HepG2 cells, have proven to be useful models that enabled the exploration of different aspects of the influence of PRDX6 that are critical to regulation of the epithelial–mesenchymal transition, which has a role in cancer progression and tumor metastasis. This evidence concerns the gene PRDX6 and neoplasm.