Subsequently, IHC and HE staining were performed to verify the expression of four genes (KRT7, KRT19, IGF2BP3 and CXCL5) in clinical PC tissues and corresponding adjacent tissues, and WB and IF also suggested that model genes were higher expressed in PANC-1 and PL45 than in hTERT-HPNE. The gene discussed is CXCL5; the disease is pachyonychia congenita.