Several strategies have been developed to study ED in cardiovascular diseases, ranging from non-invasive assessment of endothelial function with flow-mediated dilatation (FMD) of the brachial artery to measurement of peripheral blood biomarkers of ED—such as pro-inflammatory molecules, soluble adhesion molecules, and molecules that participate in the coagulation pathway such as von Willebrand factor (vWF) and thrombomodulin—as they represent a simple and non-invasive method for assessing the integrity of endothelial function [33]. This evidence concerns the gene VWF and cardiovascular disorder.