After knockdown of SMC1A, the proliferation and sphere-forming ability of PCa DU145 and PC-3 cells decreased, and they became more sensitive to X-ray treatment, which was related to reversing the EMT phenotype and downregulating the stem cell markers (CD44, LEF-1, and POU5F-1) of PCSCs. This evidence concerns the gene CD44 and posterior cortical atrophy.