The in vivo CircPSD3 overexpression induced by AAV8-circPSD3 injection inhibits HSCs activation and proliferation and alleviates CCl4-induced HF, which can be evidenced by the decreased serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels, collagen deposition, liver hydroxyproline content, along with pro-inflammatory cytokine and pro-fibrogenic gene levels [12]. This evidence concerns the gene GPT and hydrops fetalis.