Analysis of differentiated ALI-HNEC (human nasal epithelial cells) and –BAL of EA patients by immunofluorescence imaging showed the presence of β-tubulin IV+ ciliated and MUC5AC+ goblet cells in both nasal as well as bronchial cultures after 18 days of differentiation (Figure 2A). The gene discussed is MUC5AC; the disease is Esophageal atresia.