MGMT and glioblastoma: By determining the methylation status of 60 CpGs in 54 GBM samples, Everhard et al. found hypermethylation of six single CpGs (CpGs 27, 32, 73, 75, 79, and 80) and two CpG regions (CpGs 32–33 and CpGs 72–83) to be associated with low MGMT gene expression [44].