According to the HTMT hypothesis, the transfer of HLA proteins is particularly important in the context of immune tolerance; in fact, whole-genome sequencing of DNA isolated from BRCA-1 KO fibroblasts (see above), before and after their transformation in colon cancer cells by colon cancer EVs, showed that gene variants codifying for the extra-membrane portion of HLA proteins were transferred through EVs [153,179]. Here, BRCA1 is linked to colonic neoplasm.