Multiple CF cell lines have been generated using the Cas9 nucleases, including Calu-3 CF cells [120], F508del-CF HL-60 cells [121], F508del-CF T84 cells [122], 16HBE14o- CF cells with F508del, G542X or W1282X mutations [123], CFTR-/- IPEC-J2 porcine cell line [117], and G551D-CF Caco-2 cells [124]. The gene discussed is CFTR; the disease is cystic fibrosis.